Technical Data

T8425-01B
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
3H285
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Triiodothyronine (T3)

Thyroid hormones, thyroxine (T4) and triiodothyronine (T3) are crucial for mental development in infants, body growth in young children, and metabolism in adults. While lower thyroid hormone level (hypothyroidism) causes abnormity in childhood development and low metabolism rate in adults, higher thyroid hormone level (hyperthyroidism) results in hypermetabolic symptoms including weight loss, increased heart rate, anemia and irregular heartbeats. T4 and T3 are secreted in thyroid gland in response to the stimulation by thyroid stimulating hormone (TSH). T4 level in blood is higher than T3 since T4 is produced more in thyroid and is more stable. T3 is more active and it is mostly derived from conversion of T4 by deiodinases in liver, gut, and in other organs or tissues. T3 and T4 exist in blood in active free forms and also bind with proteins to facilitate transportation.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
T3-BSA conjugate.
Form
Supplied as a liquid in PBS, pH 7.2
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human triiodothyronine (T3).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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