Goat Anti-TRIM (T Cell Receptor-Interacting Molecule)
Trat1, T cell Receptor-associated Transmembrane Adaptor 1, pp29/30
Human TRIM (T cell receptor–interacting molecule) (also trat1; T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 27-30kD, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8 amino acid (aa) extracellular region, a 19 aa transmembrane region, and a 159 aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM.
Applications
Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 25ug/ml use 10ul to label 10e6 cells. Direct ELISA: 0.5-1.0ug/ml detection limit for rhTRIM is ~ 0.3ng/well. Western Blot: 1.0ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived rhTRIM. (aa 49 - 186)
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TRIM.
