Technical Data

T8663-70C
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_001596
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-tRNA Synthetase, Alanyl, NT (AlaRS, Alanyl-tRNA Synthetase, Cytoplasmic, Alanine-tRNA Ligase, AlaRS, Renal Carcinoma Antigen NY-REN-42, AARS)

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa68-98 from the N-terminal region of human AARS.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human AARS.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Shiba,K.,Biochemistry 34(33), 10340-10349 (1995). 2.Ripmaster,T.L.,Proc. Natl. Acad. Sci. USA 92(11), 4932-4936 (1995) 3.Sang Lee,J.,Cell 127(3), 635-648 (2006).
USBio References
No references available
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