Mouse Anti-Troponin I, Cardiac, aa169-178 (cTnI)
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character.
The tissue specific subtypes are
Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042) Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043) Cardiac troponin I, TNNI3 (19q13.4, 191044)
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Matched Pair
Suitable for use as the detection or conjugate (label) antibody. Matched to T8665-16E as the capture or solid phase antibody.
Also suitable as the detection or conjugate (label) antibody with T8665-19B. Matched to T8665-16E and T8665-16H, T8665-16R as the capture or solid phase antibodies.
Hybridoma
Hybridization of Sp 2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Free human Cardiac Troponin I
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human cTnI between aa169-178. Reacts equally with free cardiac troponin I (cTnl) and cTnI forming complexes with other troponin components (in the presence of 5mM EDTA). Not affected by heparin, phosphorylation and oxidation. No crossreactivity with skeletal muscle troponin I.
