Technical Data

T9154-01
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Isotype
IgM
Clone Number
5F131
Grade
Supernatant
Applications
E IF IHC WB
Crossreactivity
Ch Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Tubulin

Tubulin is found in the microtubules which represent the main structural components of centriols, the mitotic spindle, cilia and flagella.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Frozen): 1:5-1:10. Optimal dilutions to be determined by the researcher.
Positive Control
Cultured fibroblasts (chicken)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Isolated from chicken muscle cells.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Supernatant
Specificity
Recognizes chicken tubulin microtubules in muscle and non- muscle cells. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Bläckström B and Collins VP: Cytoskeletal changes in axons of rats exposed to 2,5-hexamediol, demonstrated using monoclonal antibodies. Neurotoxicology Vol. 8, 1:85-96 (1987)
USBio References
No references available
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