Technical Data

T9154-01C
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
10E52(D20G3)
Grade
Purified
Applications
IF IP WB
Crossreactivity
Hu Mk Mo Rt Ze
Gene ID
10376
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). The Elongator complex catalytic subunit (Elp3) acetylates alpha-tubulin at Lys40 while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-transcriptional modification may be required for dynamic cell shape remod-eling, cell motility, tubulin stability and terminal branching of cortical neurons (2,3).

Applications
Suitable for use in Immunofluorescence, Immunoprecipitation, Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:800 Immunoprecipitation: 1:50 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A synthetic acetylpeptide corresponding to residues surrounding Lys40 of human alpha-Tubulin. Species sequence homology: Xenopus
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of human alpha-Tubulin only when acetylated at Lys40, MW: ~52kD. Species Crossreactivity: mouse, rat, monkey and zebrafish.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Westermann, S. & Weber, K., Nat. Rev. Mol. Cell Biol. 4: 938-947 (2003). 2. Creppe, C., et al., Cell 136: 551-564 (2009). 3. Hubbert, C., et al., Nature 417: 455-458 (2002).
USBio References
No references available
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