T9154-10-FITC
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
FITCIsotype
IgG2bClone Number
6A205Grade
Affinity PurifiedApplications
IF WBCrossreactivity
Hu Mo RtShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Mouse Anti-Tubulin, alpha, acetylated (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (FITC)
The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. Stable microtubules (MTs) are known to contain acetylated alpha-tubulin. Acetylated tubulin is found in the dense networks and bundles of microtubules in the sensory and supporting cells. MTs containing acetylated alpha-tubulin in Ni2+-treated cells were resistant to disassembly induced by nocodazole, and at least partially resistant to cold temperature (0 degrees Celsius), which also depolymerizes MTs. Since acetylated alpha tubulin serves as a marker for the presence of stable MTs, the marked enhancement of alpha tubulin acetylation in Ni2+-treated cells indicates that stabilization of MTs may be an important mechanism by which Ni2+ induces cell injury since stabilized MTs in turn should favor MT bundling. The acetylated microtubules have been shown to be more drug resistant than nonacetylated microtubules. This increased stability is most likely due to the acetylation process.
Applications
Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions
Western Blot (Human, mouse, rat): 1-3ug/ml Immunofluorescence (Human, mouse): 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A chromatography
Specificity
Recognizes the ~55kD acetylated form of alpha-tubulin. |Species Crossreactivity: human, mouse and rat. Reactivity was confirmed with NIH3T3 and HeLa cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Shi Q, et al. Curr Pharm Des 4(3): 219-248,(1998). 2. Li W, et al. Toxicol Appl Pharmacol 140(2): 461-470, (1996). 3. Ogata Y, Slepecky NB. Hear Res 86(1-2): 125-131, (1995). 4. Thyberg J, Mosalewski S. Cel Tissue Res 273(3): 457-466, (1993). 5. LeDizet M, Piperno G. J Cell Biol 103(1): 13-22, (1986). 6. Caron JM, et al. Nature 317(6038): 648-651, (1985). 7. Piperno G, Fuller MT. J Cell Biol 101(6):2085-2094, (1985). 8. Piperno G, et al. J Cell Biol 104(2):289-302, (1987).USBio References
No references available