T9155-10
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
2Q121 (TU-20)Grade
AscitesApplications
E IC IHC IP WBCrossreactivity
Av Bo Hu Mk Mo Po Rt ShAccession #
NP_006077Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4)
Class 3 beta tubulin (beta 3-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class 3 beta tubulin is associated with the resistances of microtubule- targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.
Applications
Suitable for use in Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions
Immunocytochemistry (Fixed): 1:100-1:500. Immunohistochemistry (FFPE): 1:20-1:100. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Western Blot: 1:1000. Optimal dilutions to be determined by the researcher.
Positive Control
Brain tissue or neurons of any type.
Negative Control
Non-neuronal tissue or Astrocytes.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acids 443-450, ESESQGPK, of human class 3 beta-tubulin conjugated to KLH. Epitope: C-terminus.
Form
Supplied as a liquid in PBS, 1% BSA, 15mM sodium azide.
Specificity
Recognizes the beta 3 isoform of human tubulin at ~50kD which is specific for neurons. Species Crossreactivity: mouse, avian, porcine, rat, bovine, sheep and monkey. Does not identify beta tubulin found in glial cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Fernandez, M., et al., PNAS USA 101(46): 16363-16368 (2004). 2. Liour, S., Yu, R.K., GLIA 42(2): 109-117 (2003). 3. Mitsui, K., et al., J. Neuroscience 22: 9267-9277 (2002). 4. Ehtesham, M., et al., Cancer Research 62: 5657-5663 (2002). 5. Cowan, C., et al., Journal of Neuroscience 21: 7099-7109 (2001). 6. Duittoz, A.H., Hevor, T., J. Neuroscience Methods 107: 131-140 (2001). 7. Draberova, E., et al., Cell Biol. 109: 231-239 (1998).USBio References
No references available