Mouse Anti-Tubulin, gamma (TUBG, Tubulin gamma 1 Chain, TUBG1, Tubulin gamma 2 Chain, TUBG2, GCP 1, Xgam)
g-tubulin is a 51kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. g-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with a-tubulin, paxillin, and RAC GTPase activated protein 1.
Applications
Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml (1:500-1:1000) Immunocytochemistry: 2.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide corresponding to Tubulin, gamma.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes Tubulin, gamma. Species Crossreactivity: human, mouse and rat.
