Mouse Anti-TBCA (Tubulin folding cofactor A)
Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest.
Applications
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunofluorescence (IC): 1;100 Flow Cytometry: 2-5ug/ml to label 1x10e6 cells Optimal dilutions to be determined by the researcher.
Hybridoma
F0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1-108 from human TBCA, expressed in E. coli.
Form
Supplied as a liquid In PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human TBCA.
