Mouse Anti-Tumor Necrosis Factor alpha (TNFa)
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Applications
Suitable for use in ELISA and Neutralization. Other applications not tested.
Recommended Dilution
Neutralizing: Neutralizes TNF-alpha activity in vitro, 2-5ng of rTNF-alpha per ug of MAb.
ELISA: Matched pair antibodies are available
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human TNF-a.
Form
Supplied as a lyophilized powder in 0.01M PBS, pH 7.0. Reconstitute with 500ul sterile ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes both recombinant and native human TNF-a.
