Goat Anti-Tumor Necrosis Factor alpha (TNFa)
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Applications
Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested.
Recommended Dilution
ELISA: 0.5ug/ml (100ul/well antibody solution). Detection of 0.2-0.4ng mTNFa/well.
Western Blot: 0.10.2ug/ml. Detection limit is 1.5-3ng mTNFa/lane, under either reducing or non-reducing conditions.
Neutralization: To yield one half maximal inhibition (ND50) of the biological activity of mTNF-alpha (0.25ng/ml), a concentration of 0.05-0.08ug/ml of this antibody is required.
Optimal dilutions to be determined by the researcher.
Reconstitution
Reconstitute in sterile water to a concentration of ≥ 0.2mg/ml.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Highly purified (> 98%) murine TNFa.
Form
Supplied as a lyophilized powder in PBS, pH 7.2.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Tumor Necrosis Factor (TNFa) (MW 17.3kD)
