Technical Data

T9160-28D
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgD
Clone Number
9G292 (V1q)
Grade
Affinity Purified
Applications
FC N
Crossreactivity
Hm Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Tumor Necrosis Factor Alpha (TNF-alpha)

Tumor necrosis factor-alpha, a homotrimeric 17kD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further, TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines. It also induces on its turn the production of many cytokines. Furthermore, TNF-alpha has also been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors 1 and 2. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking.

Applications
Suitable for use in Flow Cytometry and Neutralization. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50 Optimal ddilutions to be determined by the researcher.
Positive Control
Monocytes/macrophages
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cytotoxin purified from T cell
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by Protein G affinity chromatography
Specificity
Recognizes natural and recombinant mouse Tumor Necrosis Factor alpha. Does not recognize membrane bound or receptor bound TNF- alpha. Species Crossreactivity: Chinese hamster

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Echtenacher, B et al; Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis. J Immunol 1990, 145: 3762. 2. Demjen, D et al; Neutralization of CD95 ligand promotes regeneration and functional recovery after spinal cord injury. Nat Med 2004, 10: 389. 3. Gerspach, J et al; Detection of membrane-bound tumor necrosis factor (TNF): An analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243.
USBio References
No references available
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