Goat Anti-TUSC2 (Tumor Suppressor Candidate 2, C3orf11, FUS1, Fus-1 Protein, Fusion 1 Protein, LGCC, PAP, PDAP2, PDGFA-associated Protein 2, Tumor Suppressor Candidate 2)
This gene is a highly conserved lung cancer candidate gene. No other information about this gene is currently available.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA:1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking
Corresponding peptide is available for Peptide Blocking studies. See T9167-03-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Immunogen
Synthetic peptide, C-TKNGQKRAKLRRVH, from the internal region of human TUSC2.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TUSC2. Species sequence homology: mouse, rat.
