Goat Anti-UBE2F (NEDD8-conjugating Enzyme UBE2F, NEDD8 Carrier Protein UBE2F, NEDD8 Protein Ligase UBE2F, NEDD8-conjugating Enzyme 2, Ubiquitin-conjugating Enzyme E2 F, NCE2)
UBE2F (Ubiquitin-conjugating enzyme E2F), a ubiquitously expressed enzyme and member of the ubiquitin-like E2 conjugating enzyme family, which acts as a specific NEDD8 conjugating enzyme, by accepting NEDD8 from the Uba3-NAE1 (APP-BP1/Uba3) E1 complex.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RNKVDDYIKRYAR, from UBE2F, at C-terminal (NP_542409.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes UBE2F. Species sequence homology: Bovine, canine, human, mouse, porcine and rat.
