Technical Data

U1000-08A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Gene #
UBA1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Ubiquitin Activating Enzyme E1, CT (A1S9 Protein, UBE1, Ubiquitin-like Modifier-activating Enzyme 1, UBA1, A1S9T, UBE1)

UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human UBE1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human UBE1. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ayusawa, D., et al., Cell Struct. Funct. 17(2):113-122 (1992). 2. Handley, P.M., et al., Proc. Natl. Acad. Sci. USA 88(1):258-262 (1991). 3. Kudo, M., et al., Exp. Cell Res. 192(1):110-117 (1991). 4. Zacksenhaus, E., et al., Cytogenet. Cell Genet. 53(1):20-22 (1990). 5. Zacksenhaus, E., et al., EMBO J. 9(9):2923-2929 (1990).
USBio References
No references available
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