Technical Data

U1005-01D
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Ubiquitin C-Terminal Hydrolase L1 (UCH-L1)

Ubiquitin carboxyl-terminal hydrolase L1 (UCH–L1), also known as PGP 9.5, is a neuronal and neuroendocrine-specific protein that plays a role in proteosomal protein degradation, which is critical for neuronal health. UCH-L1 is also involved in p27kip1 degradation through its interaction and nuclear translocation with JAB1. UCH-L1 expression is specifically associated with non small-cell lung cancer development and it is found in serum of patients with lung cancer, indicating that it may be important in lung cancer screening and diagnosis UCH-L1 may also be a useful marker for invasive colorectal cancer. Mutations in the alpha-synuclein and Uch-L1 genes cause autosomal dominant Parkinson’s disease (PD). Derangements in the ubiquitin proteasomal pathway may play important roles in the demise of dopamine neurons in PD.

Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 0.1-1ug/ml Immunohistochemistry (Paraffin): 1-2ug/ml; For staining with formalin-fixed paraffin-embedded tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining. Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide derived from the C-terminal region of human UCH-L1. Species Sequence Homology: mouse, rat, equine; 100%
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the ~26kD human UCH-L1 protein. A faint band of unknown origin may also be observed at ~50kD. Reactivity has been confirmed with mouse and rat brain homogenates, NGP neuroblastoma lysates, A549 lung carcinoma lysates, and NCI-H69 lung carcinoma lysates. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Liu, Y., et al., Cell 111: 209-218 (2002). 2. Caballero, O.L., et al., Oncogene 21(19): 3003-3010 (2002). 3. Hibi, K., et al., Am. J. Pathol. 155(3): 711-715 (1999). 4. Brichory, K., et al., Cancer Res. 61(21): 7908-7912 (2001). 5. Yamakazi, T., et al., Clin. Cancer Res. 8(1): 192-195 (2002). 6. Chung, K.K., et al., J. Neurol. 250(Sup3): III15-24 (2003).
USBio References
No references available
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