Technical Data

U1500-70F
Clone Type
Monoclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Clone Number
D1H4
Grade
Purified
Applications
IP WB
Crossreactivity
Hu Mo
Gene ID
8408
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ULK1, phosphorylated (Ser555) (Serine/Threonine-protein Kinase ULK1, Unc-51-like Kinase 1, KIAA0722)

Two related serine/threonine kinases, UNC- 51-like kinase -1 and -2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth. Both proteins are widely expressed and contain an amino terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain (CTD). The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors such as NGF. Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGap, and syntenin. Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1. Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy, a catabolic process for the degradation of bulk cytoplasmic contents. It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy, and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking. Phosphorylation of ULK1 by AMPK at Ser555 is critical for starvation-induced autophagy, cell survival under conditions of low nutrients and energy, and mitochondiral homeostasis.

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser555 of mouse ULK1. Species Sequence Homology: rat (100%)
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of mouse ULK1, phosphorylated at Ser555. Species Crossreactivity: human when phosphorylated at Ser556

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1 .Ogura, K. et al. (1994) Genes Dev 8, 2389-400. 2. Kuroyanagi, H. et al. (1998) Genomics 51, 76-85. 3. Yan, J. et al. (1998) Biochem Biophys Res Commun 246, 222-7. 4. Yan, J. et al. (1999) Oncogene 18, 5850-9. 5. Zhou, X. et al. (2007) Proc Natl Acad Sci USA 104, 5842-7. 6. Tomoda, T. et al. (2004) Genes Dev 18, 541-58. 7. Matsuura, A. et al. (1997) Gene 192, 245-50. 8. Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74. 9. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 10. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 11. Stephan, J.S. and Herman, P.K. (2006) Autophagy 2, 146-8. 12. Okazaki, N. et al. (2000) Brain Res Mol Brain Res 85, 1-12. 13. Young, A.R. et al. (2006) J Cell Sci 119, 3888-900. 14. Kamada, Y. et al. (2000) J Cell Biol 150, 1507-13. 15. Lee, S.B. et al. (2007) EMBO Rep 8, 360-5. 16. Hara, T. et al. (2008) J Cell Biol 181, 497-510. 17. Egan, D.F. et al. (2010)
USBio References
1. Milton-Laskibar I., et al. Involvement of autophagy in the beneficial effects of resveratrol in hepatic steatosis treatment. A comparison with energy restriction. 2018. Food & Function. doi 10.1039/C8FO00930A
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