Technical Data

U1621-13
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Uncoupling Protein 2 (UCP2, SLC25A8, UCPH)

White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7; ~95% homology) mitochondrial uncoupling protein (1). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle). UCP2 may play a critical role in energy balance, body weight and thermoregulation.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Western Blot: 1-10ug/ml (ECL) Immunohistochemistry: 2-20ug/ml in formaldehyde fixed tissue. Reported to detect UCP2 in human neuroblastoma cell shy cells. Optimal dilutions to be determined by the researcher.
Recommended Control Peptide
U1621-12: Uncoupling Protein 2, Human, Control Peptide (UCP2, SLC25A8, UCPH)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a 13aa sequence between the 3rd and 4th transmembrane domain of human UCP2 conjugated with KLH. Species Sequence Homology: rat and mouse; 100%, canine, bovine and porcine; 92%
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human UCP2. No significant homology with UCP1 or UCP3-5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fleury C et al (1997) Nature Genetics 15: 269-272. 2. Flier JF and Lowell BB (1997) Nature Genetics 15: 223-224. 3. Boss O et al (1997) FEBS Lett. 408:39-42. 4. Fridell Y-W C (2005) Cell Metabol. 1:145-152. 5. Mitchell JB (2003) Free Radical Biol. Med. 34: 93-102 (WB). 6. Mark FC (2006) Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 1: 365-374 (WB). 7. Ho W-L (2005) J. Neurosci. 81: 261-268 (WB, IHC).
USBio References
No references available
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