Rabbit Anti-Urate Transporter 1 (URAT1)
Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants. Urate exists primarily as a weak acid (pKa ~5.75). Urate concentration is quite high (~200-500uM) in human due to loss of uricase gene and the presence of an effective reabsorption system. Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels. URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~40kD) is ~42% identical with OAT4. Like OAT4, it is predicted to display 12 transmembrane domains with a cytoplasmic N- and C-terminus. URAT1 is prominent in epithelial cells of proximal tubule of the renal cortex. Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene.
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. Western Blot: 1-10ug/ml using ECL. Optimal dilutions to be determined by the researcher.
Control Peptide
U2005: Urate Transporter 1, Human (URAT1) (Control Peptide)
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 11aa near the C-terminus of human URAT1 (KLH). Cellular Localization: Cytoplasmic domain.
Form
Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human URAT1. Species Sequence Homology: Mouse RST/URAT1 (54%). No significant sequence homology is observed with other OAT4 or other proteins.
