Mouse Anti-V5 Epitope Tag GKPIPNPLLGLDST (Pk-tag, Paramyxovirus, SV5)
V5 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A recombinant peptide corresponding to the PK tag fused to a class II-restricted T helper cell epitope.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
