Goat Anti-VPS37C (Vacuolar Protein Sorting-associated Protein 37C, hVps37C, ESCRT-I Complex Subunit VPS37C, PML39)
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Peptide ELISA: 1:32,000 Western Blot: 0.03-0.1ug/ml, observed in human duodenum lysates ~38kD band Immunohistochemistry (Paraffin): 3-6ug/ml Requires HIER using sodium citrate buffer pH6.0 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-ETLKDKTLQELEELQ, from human VPS37C, at the internal region of the protein (NP_060436.4).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human VSP37C.
