Mouse Anti-VAMP 2 (Vesicle Associated Membrane Protein 2, Vesicle-associated Membrane Protein 2, VAMP2, VAMP-2, Synaptobrevin 2, Synaptobrevin-2, SYB 2, SYB2, SYB-2)
VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13kD member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116aa in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa31-91), and a membrane-anchor domain (aa95-114). Over (aa1-94), human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.
Applications
Suitable for use Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1ug/ml Immunohistochemistry: 25ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
Myeloma cells with B cells from Balb/c mice.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay bufter.
Immunogen
Recombinant corresponding to aa1-94 from VAMP-2 expressed in E. coli (P63027).
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human and mouse VAMP-2.
