Rabbit Anti-Vascular Endothelial Growth Factor, Mature (VEGF-C, VEGF-A, Vascular permeability factor, VPF) (MaxLight 650)
MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Vascular endothelial growth factors (VEGFs), a group of structurally and functionally related growth factors, may play important roles in vascular system formation during embryonic development, the regulation of capillary growth in adults, and the maintenance of normal vasculature. VEGF-C is a ligand for the receptor tyrosine kinases VEGFR-3 and VEGFR, bothof which are expressed by endothelial cells. VEGF-C mRNA has been detected in human heart, placenta, muscle, ovary, and small intestine, as well as in malignant tissues. A study of 35 tumor samples detected VEGF-C mRNA expression in over 50% of tumor specimens, including breast carcinomas, squamous cell carcinomas, melanomas, sarcomas, and adenomas, with notable low-level consistency for all lymphomas examined. VEGF-C has been implicated in lymphangiogenesis, physiological angiogenesis, and the development and progression of angiogenic diseases, including the angiogenesis associated with breast cancers.
Applications
Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the internal region of the human VEGF-C protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the mature form of human VEGF-C. No cross-reactivity with other species has been detected. On Western blots, this antibody recognizes the mature form of VEGF-C at 21kD, as well as forms of VEGF-C at other points in its proteolytic processing (43 and 58/61kD). Reactivity is confirmed with human PC-3 prostate adenocarcinoma cell lysates.
