Mouse Anti-VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeablility inducing properties specific for the vascular endothelium. Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell-associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Applications
Suitable for use in Neutralization of the bioactivity of human VEGF. Other applications have not been tested.
Recommended Dilution
Neutralization: Azide removal is required prior to use. Optimal dilutions to be determined by the researcher.
Positive Control
Angiosarcoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human VEGF121. Cellular Localization: Cytoplasmic, cell surface and extracellular matrix.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human Vascular Endothelial Growth Factor (VEGF). Species Crossreactivity: Rabbit.
