V2110-18F-ML490
Clone Type
PolyclonalHost
RabbitSource
MouseConjugate
MaxLight™490Isotype
IgGGrade
Affinity PurifiedApplications
FLISA WBCrossreactivity
MoShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Rabbit Anti-VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2, VEGFR-2, FLK-1, KDR) (VEGFR2, VEGFR 2, Protein-tyrosine kinase receptor flk-1, Fetal liver kinase 1, Kinase NYK, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Vascular endothelial growth factor receptor 2) (MaxLight 490)
MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Vascular endothelial growth factor receptor-2 (VEGFR-2), also known as fetal liver kinase 1 (FLK-1) in mouse and KDR in human, is a transmembrane tyrosine kinase that plays an important role in the development of the vascular system and the vascularization of a wide variety of tumors. Binding of VEGF to VEGFR-2 leads to tyrosine phosphorylation of the dimerized receptor and subsequent phosphorylation of SH2-containing intracellular signaling proteins, including phospholipase C, Src family tyrosine kinase and phosphatidylinositol 3-kinase (PI3K) adaptor molecules, SHC, NCK, and Ras GTPase-activating protein. VEGFR-2 is expressed in cultured human umbilical vein endothelial cells (HUVEC), Hela cells, WM 35 melanoma cells, WM 9 melanoma cells, as well as mouse NIH3T3, and Balb/c 3T3 cells. In a single study of 115 paraffin-embedded cancer specimens analyzed by immunohistochemistry, VEGFR-2 expression was observed in bladder tumors, breast cancer, intestinal cancer and lung cancer, suggesting a direct role for VEGF as an autocrine growth factor in a variety of human cancers.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 0.1-1ug/ml Western Blot: 1–5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the N-terminal region of the mouse vascular endothelial growth factor receptor-2 (VEGFR-2, FLK-1, KDR) protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Rrecognizes mouse VEGF Receptor-2. Identifies the ~230kD mature form of VEGFR-2. Reactivity is confirmed with mouse SVEC 4–10 cell lysates. Human reactivity is expected based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Botta, R.M., et al., Exp. Hematol. 28(12): 1501 (2000). 2. Chiang, M.K., et al., Growth Factors 12(1): 1-10 (1995). 3. Heidenreich, R., et al., Cancer Res. 60(21): 6142-6147 (2000). 4. Dayanir, V., et al., J. Biol. Chem. 276(21): 17,686-17,692 (2001). 5. Soker, S., et al., J. Biol. Chem. 271(10): 5761-5767 (1996). 6. Terman, B., et al., Growth Factors 11(3): 187-195 (1994). 7. Waltenberger, J., et al., J. Biol. Chem. 275(21): 15,905-15,911 (2000). 8. Tian, X., et al., Biochem. Biophys. Res. Commun. 286(3): 505-512 (2001).USBio References
No references available