May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 0.3-1ug/ml, observed in human adipose lysates pn ~48kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RARWKHEFDPNVTK, from human SERPINA12, at the internal region of the protein (NP_776249.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SERPINA12.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Klöting N, Berndt J, Kralisch S, Kovacs P, Fasshauer M, Schön MR, Stumvoll M, Blüher M. Vaspin gene expression in human adipose tissue: association with obesity and type 2 diabetes. Biochem Biophys Res Commun. 2006 Jan 6;339(1):430-6. Epub 2005 Nov 15.
USBio References
No references available
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