V2121-15J
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Ca HuAccession #
NP_006104.4, NP_001073343.1Gene ID
10451Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-VAV3 (Vav 3 Guanine Nucleotide Exchange Factor, VAV-3, FLJ40431, Guanine Nucleotide Exchange Factor VAV3)
Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization. Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 0.3-1ug/ml, observed in human bone marrow lysates on ~80kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to CSGEQGTLKLPEK, from human VAV3, at internal region of the protein (NP_006104.4, NP_001073343.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human VAV3. Species sequence homology: Canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Salhia B, Tran NL, Chan A, Wolf A, Nakada M, Rutka F, Ennis M, McDonough WS, Berens ME, Symons M, Rutka JT. The guanine nucleotide exchange factors trio, Ect2, and Vav3 mediate the invasive behavior of glioblastoma. Am J Pathol. 2008 Dec;173(6):1828-38.USBio References
No references available