V2121-16D
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
Hu MoShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Vav3 (vav 3 oncogene, Oncogene VAV23 Guanine nucleotide exchange factor, Gef, GDP/GTP Exchange Factor, Gef)
Vav proteins belong to the guanidine nucleotide exchange factor family of proteins. These proteins couple receptors to Rho-GTPases. To date, three members of the Vav family have been identified in mammalian cells: Vav1, Vav2 and Vav3. Vav proteins contain multiple protein binding motifs and act as adaptor proteins linking cell surface receptors to downstream signaling proteins. Vav3 transcripts are ubiquitously expressed in wide variety of cell lines including hematopoietic cells, suggesting its role in lymphocyte development and antigen receptor mediated activation of NFAT and NFkB. Vav3 is also involved in activating JNK, p21-activated kinase (PAK) and PLCy, implicating a critical role in cytoskeletal rearrangement. Vav3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of Vav3.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Cos7 cells overexpressing Vav3, +/- epidermal growth factor (EGF.)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide derived from a region of human Vav3 that contains tyrosine 173. Species sequence homology: mouse.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Vav3. Species sequence homology: Human (100%) and chicken (92%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Sachdev, P., et al. (2002) Distinct role of phosphatidylinositol 3-kinase and Rho family GTPases in Vav3-induced cell transformation, cell motility, and morphological changes. J. Biol. Chem. 77(20):17638-17648. 2. Fujikawa, K., et al. (2002) Vav3 is regulated during the cell cycle and effects cell division. Proc. Nat’l. Acad. Sci. USA 99(7):4313-4318. 3. Zeng, L., et al. (2000) Vav3 mediates receptor protein tyrosine kinase signaling, regulates GTPas activity, modulates cell morphology, and induces cell transformation. Mol. Cell. Biol. 20(24):9212-9224. 4. Costello, P.S., et al. (1999) The Rho-family GTP exchange factor Vav is a critical transducer of Tcel receptor signals to the calcium, ERK, and NF-kappaB pathways. Proc. Nat’l. Acad. Sci. USA. 96(6):3035-3040. 5. Movilla, N. and X.R. Bustelo (1999) Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins. Mol. Cell. Biol. 19(11):7870-7885.USBio References
No references available