V2122-01R
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
VI-RE/1Grade
Affinity PurifiedApplications
E FC IC IHC WBCrossreactivity
HuGene ID
7431Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-Vimentin (VIM)
Vimentin is the major subunit protein of the intermediate filaments of mesenchumal cells, and has a molecular weight of 57kD. Immunohistochemical staining for Vimentin is thus characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of malignant tumors, generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments. The antibody stains the 57kD vimentin band in immunoblots performed on a lysate of normal human lymphocytes. It does not stain mouse or rat vimentin.
Applications
Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunohistochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1-4ug/ml using intracellular staining Western Blot: 1-2ug/ml Immunocytochemistry: 5-10ug/ml Immunohistochemistry: paraffin sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Bacterially expressed full-length human vimentin
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human vimentin. Does not react with mouse or porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Chen YK, Chang WS, Wu IC, Li LH, Yang SF, Chen JY, Hsu MC, Chen SH, Wu DC, Lee JM, Huang CH, Goan YG, Chou SH, Huang CT, Wu MT: Molecular characterization of invasive subpopulations from an esophageal squamous cell carcinoma cell line. Anticancer Res. 2010 Mar; 30(3):727-36.USBio References
No references available