Technical Data

V2640-03
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Von Hippel-Lindau Protein (vHL)

von Hippel-Lindau (VHL) disease is a hereditary cancer with a predilection for the central nervous system and retina. The von Hippel-Lindau tumor suppressor gene is mutated in families with von Hippel-Lindau disease and encodes a protein (VHL) of 213 amino acids with an acidic pentapeptide motif in the N-terminus. Mutations in the VHL gene result in constitutive expression of many hypoxi-induced genes, at least in part because of increases in the cellular level of hypoxia-inducible transcription factor HIF-1α. VHL protein binds to elongin B, elongin C, and Cul2 to form a stable complex that targets hypoxia inducibal factors (HIFs) for degradation and transcriptional regulation. In addition, VHL protein has also been shown to interact with specific protein kinase C isoforms, histone deacetylases and HIF-1 inhibitor (FIH-1). α-VHL is an affinity-purified rabbit polyclonal antibody raised against the recombinant VHL protein.

Applications
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:200-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50?. Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant VHL |protein.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
As reported
Specificity
α-VHL reacts with the VHL protein in HeLa nuclear extract by Western blotting.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Singh et al. (2001) Survey of Ophthalmology 46:117. |Latif et al. (1993) Science 260:1317. |Linehan et al. (1995) J. Am. Med. Assoc. 273:564. |Duan et al. (1995) Proc. Natl. Acad. Sci. USA 92:6459. |Kamura et al. (2000) Proc. Natl. Acad. Sci. USA 97:10430. |Duan et al. (1995) Science 269:1402. |Iwai et al. (1999) Proc. Natl. Acad. Sci. USA 96:12436. |Pal et al. (1997) J Biol. Chem. 272:27509. |Mahon et al. (2001) Genes & Dev. 15:2675. |
USBio References
No references available
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