Mouse Anti-WDHD1 (WD Repeat and HMG-box DNA-binding Protein 1, Acidic Nucleoplasmic DNA-binding Protein 1, And-1, And1)
AND-1 (WDHD1) is also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 2ul/ml Immunohistochemistry (formalin-fixed paraffin-embedded tissue): 15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant protein corresponding to the C-terminal region of AND-1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes AND-1. Species Crossreactivity: human and mouse.
