Mouse Anti-WNK2 (Serine/threonine-protein Kinase WNK2, Protein Kinase With No Lysine 2, Protein Kinase Lysine-deficient 2, Serologically Defined Colon Cancer Antigen 43, Antigen NY-CO-43, KIAA1760, PRKWNK2, SDCCAG43) (Azide Free) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
The protein encoded by this gene is a cytoplasmic serine-threonine kinase that contains cysteine in place of the lysine found at the conserved ATP-binding location in subdomain II of protein kinases. Since this protein does have kinase activity, it is possible that another lysine in the kinase subdomain I can substitute for the missing conserved lysine. [provided by RefSeq]
Applications
Suitable for use in FLISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 1.5ug/ml Western Blot: 1:500-1:1,000 FLISA: 1ug/ml-3ng/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to a portion of amino acids within aa2118-2218 of human WNK2.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human WNK2.
