Technical Data

Y3051-02
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_057066
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-YBX2, CT (Y-box-binding Protein 2, Germ Cell-specific Y-box-binding Protein, Contrin, MSY2 Homolog, DNA-binding Protein C, Dbpc)

YBX2 is a major constituent of messenger ribonucleoprotein particles (mRNPs). It is nvolved in the regulation of the stability and/or translation of germ cell mRNAs. It binds to Y-box consensus promoter element and to full length mRNA with high affinity in a sequence-independent manner. It binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. YBX2 may mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human YBX2 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human YBX2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kohno,Y., Br. J. Cancer 94(5), 710-716 (2006). 2. Yoshida,T., Biochim. Biophys. Acta 1759(1-2), 80-88 (2006). 3. Tekur,S., J. Androl. 20(1), 135-144 (1999). 4. Gu,W., Biol. Reprod. 59(5), 1266-1274 (1998).
USBio References
No references available
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