Rabbit Anti-ZBP1 (Z-DNA Binding Protein 1, C20orf183, DAI, dJ718J7.3, DLM1, DLM-1, Tumor Stroma and Activated Macrophage Protein DLM-1, DNA-dependent activator of IRFs) (MaxLight 550)
DNA-dependent activator of IRFs
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Initial studies suggested that DAI may be involved in processes such host response to neoplasia, additional research has indicated that the cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).
DAI is highly expressed in lymphatic tissues such as the lymph node, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seem in thymus, lung and liver. It has been demonstrated that multiple isoforms of DAI exist.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to an 18aa sequence from near the carboxy terminus of human ZBP1.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DAI. Species Crossreactivity: mouse and rat.
