Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, ZIP4, Zip4, Zip5, LIV-1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types. Zip4 (SLC39A4) belongs to the LZT subfamily. There are two hZIP4 isoforms, which only differ in their amino and carboxy termini. The largest isoform encodes a 647aa protein with an N-terminal signal peptide, whereas the shorter isoform has only 626aa.
ZIP4 rat: 656aa; human: 647aa; mouse: 660aa - 71kD; Chromosome: 15D3. It is highly expressed in the small intestine and embryonic visceral yolk sac; weakly expressed in the stomach and liver. Found to the apical surface of enterocytes and visceral endoderm cells during zinc deficiency.
Applications
Suitable for use in Western Blot, ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml (Chemiluminescence) ELISA: 1:100,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 18aa of mouse ZIP4 (Accession #Q78IQ7), N-terminal, Extracellular domain (KLH).
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ZIP4. Species sequence homology: rat (95%).