Mouse Anti-ZNF143 (Zinc Finger Protein 143, SPH-binding Factor, Selenocysteine tRNA Gene Transcription-activating Factor, hStaf, SBF, STAF)
ZNF143 is a 69kD (unglycosylated) member of the GLI C2H2-type zinc-finger protein family. Human ZNF143 is 638aa in length. A second isoform is produced from the deletion of residues 39-69. The protein contains seven C2H2-type zinc fingers, and is expressed in all tissues tested with greatest expression in the ovary. Functionally, ZNF143 serves as a transcriptional activator. Its other functions include acting as an activator for the gene for selenocysteine tRNA (tRNAsec), binding to the SPH motif of small nuclear RNA (snRNA) gene promoters, and participating in efficient U6 RNA polymerase III transcription through its interaction with CHD8. Human ZNF143 shares 97% aa sequence identity with mouse ZNF143.
Applications
Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa459-638 from human ZNF143 expressed in E.coli.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human ZNF143. Does not react with recombinant human ZNF24, rhZNF206, rhZNF281, rhDC-SCRIPT/ZNF366, rhZNF423, or recombinant mouse Bcl-6.
