034957-FITC
Grade
Affinity PurifiedGene ID
1950Gene #
EGFAccession Number
NP_001954.2EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
EGF, CT (EGF, Pro-epidermal growth factor, Epidermal growth factor, Urogastrone) (FITC)
EGF, Pro-epidermal growth factor, Epidermal growth factor, Urogastrone
This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.
Applications
Suitable for use in Western Blot, FLISA
Recommended Dilution
FLISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
de Diesbach, M.T., et al. Exp. Cell Res. 316(19):3239-3253(2010)|Xu, Z., et al. Biochem. Biophys. Res. Commun. 401(3):376-381(2010)|Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)|Hommel, U., et al. J. Mol. Biol. 227(1):271-282(1992)|Hernandez-Sotomayor, S.M., et al. J. Membr. Biol. 128(2):81-89(1992)USBio References
No references available