Brain Natriuretic Peptide (BNP) Recombinant, Canine
Source
Recombinant Canine from E. coli
Endotoxin
1.0EU per 1ug (determined by the LAL method)
Fragment
His18~Arg106 (Accession No: P16859)
Sequence
MGHHHHHHSGSEF-HLS PLGGRPHPLG GRSPASEASE ASEASGLWAV QELLGRLKDA VSELQAEQLA LEPLHRSHSP AEAPEAGGTP RGVLAPHDSV LQALRR
Epitope Tag
N-terminal His-tag
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. Aliquots are stable for at least 12 months from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Source
Recombinant, E. coli
Form
Supplied as lyophilized powder in PBS, pH7.4, 5% sucrose, 0.01% sarcosyl. Reconstitute in sterile PBS, pH7.2.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
