Technical Data

171825
Grade
Highly Purified
Accession Number
NM_013229
Molecular Weight
143
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C
APAF1, Recombinant, Human (Apoptotic peptidase activating factor 1)

Human apoptotic peptidase activating factor 1 (APAF1), also known as CED4, (GenBank Accession No. NM_013229), a.a.2-1237(end) with N-terminal His-tag and N-terminal FLAG-tag, MW=143kD, expressed in a baculovirus infected Sf9 cell expression system.

Source
Recombinant protein corresponding to aa2-1237 from APAF1 at N-terminus, fused to His-tag and Flag-tag expressed in a baculovirus infected Sf9 cell expression system.
Molecular Weight
~143kD
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
Source
Recombinant, Baculovirus
Purity
~90%
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween 20, 80ug/ml FLAG peptide, 20% glycerol
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Yuan, S., et al., Biochemistry. 2013 Apr 2;52(13):2319-27 2. Saleh, A., et al., J Biol Chem. 1999 Jun 18;274(25):17941-5
USBio References
No references available
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