Technical Data

207133
CAS Number
81552-33-2
Grade
Highly Purified
Molecular Formula
C45H73NO14
Molecular Weight
852.1
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
Concanamycin B (High Purity) (8-Deethyl-8-methylconcanamycin A)

Antibiotic. Exhibits similar activity as concanamycin A and concanamycin C. Specific vacuolar-type H+-ATPase inhibitor. Inhibitor of autophagic degradation by rising lysosomal pH and thus inactivating the lysosomal acid hydrolases. Suppresses bone resorption. Inhibits proliferation of mouse splenic lymphocytes. Antifungal and larvicidal.

CAS Number
81552-33-2
Source
Streptomyces sp.
Formula
C45H73NO14
Solubility
DMSO, chloroform, acetonitrile or methanol; insoluble in H2O.
Molecular Weight
852.1
Method for Determining Identity
Proton NMR
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Streptomyces sp.
Purity
≥98% (HPLC)
Form
Supplied as a white to off-white solid.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Structures of concanamycins B and C: H. Kinashi, et al.; J. Antibiot. 35: 1618 (1982). 2. Isolation and characterization of concanamycins A, B and C: H. Kinashi, et al.; J. Antibiot. (Tokyo) 37: 1333 (1984). 3. Involvement of the vacuolar H(+)-ATPases in the secretory pathway of HepG2 cells: M. Yilla, et al.; J. Biol. Chem. 268: 19092 (1993). 4. Inhibitory effect of modified bafilomycins and concanamycins on P- and V-type adenosintriphosphatase: S. Drose, et al.; Biochemistry 32: 3902 (1993). 5. Characterization of the ATPase activity of P-glycoprotein from multidrug-resistant Chinese hamster ovary cells: F.J. Sharom, et al.; Biochem. J. 308 (Pt2): 381 (1995). 6. Concanamycin B inhibits the expression of newly synthesized MHC class II molecules on the cell surface: K. Ito, et al.; J. Antibiot. 48: 488 (1995) 7. Concanamycin B, a vacuolar H(+)-ATPase specific inhibitor suppresses bone resorption in vitro: J.T. Woo, et al.; Biol. Pharm. Bull. 19: 297 (1996). 8. In vivo rapid reduction of alloantigen-activated CD8+ mature cytotoxic T cells by inhibitors of acidification of intracellular organelles, prodigiosin 25-C and concanamycin B: M. H. Lee, et al.; Immunology 99: 243 (2000). 9. Y. Sasazawa, et. al.; Cancer Sci. 100: 1460 (2009). 10. Inhibitors of the V0 subunit of the vacuolar H+-ATPase prevent segregation of lysosomal- and secretory- pathway proteins: J. A. Sobota, et. al.; J. Cell Sci. 122: 3542 (2009) 11. Inhibitors of V-ATPase: old and new players: M. Huss, et. al.; J. Exp. Biol. 212: 341 (2009)
USBio References
No references available
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