Technical Data

214910-ML650
Grade
Affinity Purified
Gene ID
281237
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
IFN gamma (Interferon gamma) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Interferon Gamma (IFNy), recognizes the 19.3kD protein belonging to the type II interferon family.
Applications
Suitable for use in FLISA, ELISpot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0/AG-14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Walravens, K. et al. (2002) Analysis of the antigen-specific IFN-gamma producing T-cell subsets in cattle experimentally infected with Mycobacterium bovis. Vet. Immunol. Immunopathol. 84: 29-41. 2. Lemaire, M. et al. (2000) Effects of bovine Herpesvirus type 1 infection in calves with maternal antibodies on immune response and virus latency. J. Clin. Microbiol. 38: 1885-1894. 3. Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1+ gammadelta T cells. Infect. Infect Immun. 70: 1488-1500. 4. Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle. Vet. Immunol. Immunopathol. 66: 309-320.
USBio References
No references available
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