IgG, Fc (Agarose)
Chickens were immunized with purified Human IgG. Antibodies were isolated from immune eggs, affinity purified on a human IgG Fc column and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
Applications
Suitable for use in Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Specific Activity
1.2mg human IgG Fc bound per ml agarose.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: We do not recommended adding glycerol as a cryopreservative.
