Technical Data

351748
CAS Number
14892-97-8
Grade
Highly Purified
Molecular Formula
C₁₈H₁₂N₄OS
Molecular Weight
332.38
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C
SCR7 Pyrazine
2,3-Dihydro-6,7-diphenyl-2-thioxo-4 (1H)-pteridinone; 6,7-Diphenyl-2-thio-lumazine

SCR7 pyrazine also is a potent inhibitor of nonhomologous endjoining (NHEJ) mediated by DNA ligase IV. It enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro upt to 19-fold. SCR7 pyrazine is a product of spontaneous cyclization of CRISPR enhancer SCR7 first reported by Srivastava, M., et al.

DNA ligase IV seals double-strand breaks during the process of nonhomologous end-joining in DNA repair. Inhibiting this function in cancer cells is one strategy to prevent deleterious cell growth. SCR7 is a small molecule inhibitor of DNA ligase IV that prevents nonhomologous end-joining by interfering with ligase binding and activating apoptosis.1 It also inhibits ligase III, but does not affect the activity of T4 DNA ligase or ligase I. SCR7 has been used to increase the rate of homology directed repair triggered by DNA double-strand breaks and to inhibit cancer cell growth in vitro (IC50s = 8-120 µM) and in mouse models when co-administered with double-strand break-inducing therapeutic compounds.1,2,3,4
Synonyms
2,3-Dihydro-6,7-diphenyl-2-thioxo-4 (1H)-pteridinone; 6,7-Diphenyl-2-thio-lumazine
CAS No
14892-97-8
Molecular Formula
C₁₈H₁₂N₄OS
Molecular Weight
332.38
Appearance
Supplied as a yellow solid.
Purity
≥95%
Solubility
Soluble to 100mM in DMSO and to 20mM in ethanol
Storage and Stability
Store at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Chu et al (2015) Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat.Biotechnol. 33: 543. PMID: 25803306. 2. Maruyama et al (2015) Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining. Nat.Biotechnol. 33: 538. PMID: 25798939.
USBio References
No references available
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