A2230
CAS Number
1397-89-3Grade
Cell Culture GradeMolecular Formula
C47H73NO17Molecular Weight
924.09EU Commodity Code
38220090Shipping Temp
Blue IceStorage Temp
-20°CAmphotericin B Solution, 100X
Abelcet; Abelecet; AmBisome; Ampho-Moronal; Amphocin; Amphozone; Apothecon; Fungilin; Fungizona; Fungizone; Halizon; LNS-AmB; NS 718; NSC 527017
Amphotericin B is a polyene antifungal agent for yeast, mold and mycoplasma. It was first isolated by Gold, et a., from Streptomyces nodosus in 1955. It is an amphoteric compound composed of a hydrophilic polyhydroxyl chain along one side and a lipophilic polyene hydrocarbon chain on the other. Amphotericin B is poorly soluble in water. Amphotericin B binds to sterols, preferentially to the primary fungal cell membrane sterol, ergosterol. This binding disrupts osmotic integrity of the fungal membrane, resulting in leakage of intracellular potassium, magnesium, sugars, and metabolites and then cellular death.
Used in the preparation of baculoviral stocks
Amphotericin B Solution, 100X is composed of USP grade Amphotericin B in a High pH buffer (NaOH/Na+,H2O). The concentration of Amphotericin B in the final product is 0.25 g/L.
Synonyms
Abelcet; Abelecet; AmBisome; Ampho-Moronal; Amphocin; Amphozone; Apothecon; Fungilin; Fungizona; Fungizone; Halizon; LNS-AmB; NS 718; NSC 527017
Molecular Formula
C47H73NO17
Appearance
Light yellow solution
Sterility
Aseptically prepared by 0.2um filtration
Working Concentration
Add aseptically 1ml concentrate to 100ml culture medium.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley (1992) 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989)|2. Asher, I.M., et al.: Anal. Profiles Drug Subs., 6, 1, (1977)USBio References
1. Pein M.,et al. CXCR3-expressing metastasis-initiating cells induce and exploit a fibroblast niche in the lungs to fuel metastatic colonization bioRxiv preprint first posted online Feb. 11, 2019; doi: http://dx.doi.org/10.1101/546952.