A3578-ML550
Grade
Affinity PurifiedEU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
ART2.2 (ADP Ribosyltransferase 2.2) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
ART2.2 is expressed exclusively by mature T-cells. Levels of ART2.2 expression vary between inbred strains of mice and is absent in NZW mice, in which the ART2.2 gene is deleted (1).
Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Hybridoma
Spleen cells from immunized wistar rats were fused with cells of the Sp2/0 mouse myeloma cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.