Technical Data

B0901-03B
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BD2 (beta Defensin-2, beta 2 Defensin, Defensin beta 2, BD 2, BD-2, DEFB2, DEFB-2, HBD 2, HBD2, HBD-2, DEFB102, Skin Antimicrobial Peptide 1, SAP1)
Beta 4 Defensin, Defensin 4 beta, Defensin beta 4, DEFB4, DEFB-4

Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-terminal active portion of the protein.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bals, R. et al. (1998) Human beta-Defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J. Clin. Invest. 102: 874-880. 2. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721. 3. Harder, J. et al. (2000) Mucoid Pseudomonas aeruginosa, TNF-a, and IL-1beta, but not IL-6, induce human beta-Defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714-721.
USBio References
No references available
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