Technical Data

C2402-04-ML490
Grade
Affinity Purified
EU Commodity Code
30021010
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
CD47 (MaxLight 490)

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilution determined by the researcher.
Hybridoma
Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Recommended Secondary Reagents
IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed)
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Vernon-Wilson, E. F. et al. (2000). CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRP alph a 1. Eur. J. Immunol. 30: 2130-2137. 2. de Vries, H.E. et al. (2002). Signal regulatory protein alpha - CD47 interactions are required for the transmigration of monocytes across cerebral endothelium. J. Immunol. 168: 5832 - 5839.
USBio References
No references available
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