C3490
CAS Number
75621-03-3Grade
Molecular Biology GradeMolecular Formula
C32H58N2O7SMolecular Weight
614.88EU Commodity Code
38220090Shipping Temp
RTStorage Temp
-20°CCHAPS ((3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate))
(3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate); N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3α,5β,7α,12α)-3,7,12-trihydroxy-|24-oxocholan-24-yl]amino]-1-propanaminium Inner Salt; CHAPS
Non-denaturing zwitterionic detergent with cholic acid and sulfobetaine moieties, used to dissolve biological membranes, useful for protein extraction methods due to its ease of removal by dialysis or gel filtration. Low background UV absorption for protein detection.
Designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes.
Synonyms
(3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate); N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3α,5β,7α,12α)-3,7,12-trihydroxy- 24-oxocholan-24-yl]amino]-1-propanaminium Inner Salt; CHAPS
Molecular Formula
C32H58N2O7S
Appearance
Supplied as a white to off white powder.
Purity
≥98% (typically ≥99%)
Solubility (10% aq)
Colorless, clear, complete. Soluble in DMF, hot Methanol, Water (50mg/1ml)
Storage and Stability
RT short-term, -20°C long-term. Hygroscopic. Keep tightly closed and desiccated.
