D1649-01B
Grade
PurifiedAccession Number
NP_054699Molecular Weight
101.127EU Commodity Code
30021010Shipping Temp
Blue IceStorage Temp
-20°CMCK10 (Epithelial Discoidin Domain Receptor 1, Tyrosine Kinase DDR, Discoidin Receptor tyrosine Kinase, Tyrosine-protein Kinase CAK, Cell Adhesion Kinase, TRK E, Protein-tyrosine Kinase 3A, Protein-tyrosine Kinase RTK-6, HGK2, CD167 Antigen-like Family Member A, Mammary Carcinoma Kinase 10, CD167a, DDR1, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE)
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain.
Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Vogel, W., et al., Mol. Cell 1(1):13-23 (1997). 2.Playford, M.P., et al., Genome Res. 6(7):620-627 (1996). 3.Perez, J.L., et al., Oncogene 12(7):1469-1477 (1996). 4.Valent, A., et al., Hum. Genet. 98(1):12-15 (1996). 5.Edelhoff, S., et al., Genomics 25(1):309-311 (1995).USBio References
No references available